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KMID : 0364820080440020164
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.164 ~ p.167
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RCA-mer: A Web-Based Program Searching for Primer Candidates
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Cho Young-Hoon
Lee Dae-Sang
Park Kie-Jung
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Abstract
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Recently, rolling circle amplification (RCA) technique has been widely focused in the field of gene amplification
just like PCR method. We have developed RCA-mer, which is a web-based program searching for
primer candidates from a given sequence. It can be applied to find primer lists in DNA amplification experiment
based on RCA method. The RCA-mer compares 8-mer primer lists with user’s input sequence such as vector,
mitochondria, and microbial genome sequence. After calculating 8-mers existences in a given sequence, it displays
matched and no-matched primer lists with their GC-contents. In addition to it, RCA-mer can search the
existence of 8-mer primer lists in two sequences whether they are co-existed or not. Users can apply candidate
primer lists to their researches which use RCA techniques.
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KEYWORD
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Fosmid, N-mer, PCR, primer, rolling circle amplification, vector
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